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Proceedings Paper

Automated filtration capture immunoelectrochemical assay of bacteria
Author(s): Jeffrey D. Brewster
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Paper Abstract

An automated system for filtration capture and immunoelectrochemical detection of bactria in liquid samples is described. The detector incorporates a porous electrode in contact with the filter, rather than the solid electrode used previously, to allow sample and reagent solutions to be delivered in a flowing stream. This eliminated the need for manual assembly of the electrode and filter for each assay and allowed repetitive assays on a single filter/electrode. The electrochemical response of the novel gold grid electrode under static and flow conditions was found to be consistent with theory for a planar electrode operating in laminar flow conditions. A computer-controlled fluid handling system was coupled to the detector for delivery of samples and reagents at controlled flow rates and times. The combination of flow detector and fluid handling system allows for automation of the previous assay protocol as well as providing new operating modes with enhanced background rejection and improved sensitivity. The use of these operating modes is demonstrated by a simple assay for Escherichia coli O157:H7 with virtually no background current.

Paper Details

Date Published: 12 January 1999
PDF: 19 pages
Proc. SPIE 3544, Pathogen Detection and Remediation for Safe Eating, (12 January 1999); doi: 10.1117/12.335767
Show Author Affiliations
Jeffrey D. Brewster, USDA Agricultural Research Service (United States)

Published in SPIE Proceedings Vol. 3544:
Pathogen Detection and Remediation for Safe Eating
Yud-Ren Chen, Editor(s)

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