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Proceedings Paper

Effect of HeNe laser on calcium signals in sperm cells
Author(s): Rachel Lubart; Harry Friedmann; Natalie Cohen; Haim Brietbart
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Paper Abstract

Irradiation of mouse spermatozoa by 630 nm HeNe laser was found to enhance calcium transport in these cells. The change in Ca transport was investigated through two approaches, the first employing the fluorescent Ca indicator, Fluo-3 AM and a fluorescence microscopic system, and the second the radiolabeled Ca uptake. In both approaches the effect of light on Ca transport was abrogated in the absence of Ca during the irradiation time, indicating that the effect of light is Ca-dependent. The stimulatory effect of light on Ca uptake was inhibited by treatment with catalase, suggesting H2O2 to be involved in light stimulated Ca2+ uptake. The stimulatory effect of light on Ca uptake was abolished in the presence of a voltage-dependent Ca-channel inhibitor, nifedipine, indicating the involvement of a plasma membrane, voltage- dependent Ca-channel. In contrast, addition of nifedipine prior to the HeNe laser irradiation did not affect the light-induced rise in intracellular Ca levels, as measured with Fluo-3 loaded sperm cells. Therefore, it can be concluded that this Ca influx occurs via a voltage- insensitive Ca-channel. The stimulatory effect of light on Ca uptake was almost completely abolished by the mitochondrial uncoupler FCCP. These data imply that light affects the mitochondrial Ca transport mechanisms. It is well known that Ca influx from an extracellular environment is an essential component of a signaling cascade leading to fertilization.

Paper Details

Date Published: 29 December 1998
PDF: 5 pages
Proc. SPIE 3569, Effects of Low-Power Light on Biological Systems IV, (29 December 1998); doi: 10.1117/12.334383
Show Author Affiliations
Rachel Lubart, Bar Ilan Univ. (Israel)
Harry Friedmann, Bar Ilan Univ. (Israel)
Natalie Cohen, Bar Ilan Univ. (Israel)
Haim Brietbart, Bar Ilan Univ. (Israel)


Published in SPIE Proceedings Vol. 3569:
Effects of Low-Power Light on Biological Systems IV
Giovanni F. Bottiroli; Tiina I. Karu; Rachel Lubart, Editor(s)

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