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Proceedings Paper

Laser trapping of micron-sized objects: theory and experiment
Author(s): Libor Sramek; Alexandr Jonas; Miroslav Liska; Pavel Zemanek
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Paper Abstract

Force exerted on a microobject by the focused laser beam can be as high as the gravity force acting on the object or even significantly higher. This implies that the laser light can be used to manipulate small objects (range size approximately 0.1 - 100 micrometers ) within a suitable immersion medium. This possibility is of a great practical importance, for example, for microbiology and molecular biology (manipulation of single living cells, cell organelles, chromosomes, etc.) as well as for micromachinery and other technical branches. We use a ray-optics-based model to determine the magnitude of forces exerted by laser light as the functions of laser beam, object and surrounding medium parameters. We study the influence of these parameters on the total force in order to find the optimal parameter combination for the most effective manipulation. We have employed these theoretical results in practice and succeeded in building up a 3D laser trap which we use to manipulate divinylbenzen spherical particles (10 - 35 micrometers sized) and also irregularly shaped living protozoa cells in water medium.

Paper Details

Date Published: 6 November 1998
PDF: 9 pages
Proc. SPIE 3580, Photoconversion: Science and Technologies, (6 November 1998); doi: 10.1117/12.330459
Show Author Affiliations
Libor Sramek, Technical Univ. of Brno (Czech Republic)
Alexandr Jonas, Technical Univ. of Brno (Czech Republic)
Miroslav Liska, Technical Univ. of Brno (Czech Republic)
Pavel Zemanek, Institute of Scientific Instruments (Czech Republic)


Published in SPIE Proceedings Vol. 3580:
Photoconversion: Science and Technologies
Maksymilian Pluta; Mariusz Szyjer, Editor(s)

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