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Proceedings Paper

Optical MEMS-based fluorescence detection scheme with applications to capillary electrophoresis
Author(s): Kurt D. Kramer; Kwang Wook Oh; Chong Hyuk Ahn; James J. Bao; Kenneth R. Wehmeyer
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Paper Abstract

A new laser-induced fluorescence detection system for capillary electrophoresis has been designed, built, and tested in this work. Multi-mode optical fibers were incorporated on-chip to couple laser light into the detection area on a borosilicate glass substrate. A metal- semiconductor-metal GaAs photodiode was fabricated and mounted over the separation capillary using a new conductive polymer flip-chip bonding approach, replacing the traditional photomultiplier tube in the detection scheme. Proper operation of the photodiode after interconnection was verified by directly impinging light into the detector using two laser sources. For both dry and filled channels, several laser source-fluorescent dye combinations were employed in order to generate a fluorescence signal (approximately few nA) detected by the photodiode. We thus demonstrate the feasibility of integrating optical MEMS into chemical micro total analysis systems ((mu) -TAS). In the future, this has the potential to improve functionality, throughput, and ease of operation of these `laboratories on chip'.

Paper Details

Date Published: 10 September 1998
PDF: 10 pages
Proc. SPIE 3515, Microfluidic Devices and Systems, (10 September 1998); doi: 10.1117/12.322098
Show Author Affiliations
Kurt D. Kramer, Univ. of Cincinnati (United States)
Kwang Wook Oh, Univ. of Cincinnati (United States)
Chong Hyuk Ahn, Univ. of Cincinnati (United States)
James J. Bao, Proctor & Gamble Pharmaceuticals (United States)
Kenneth R. Wehmeyer, Proctor & Gamble Pharmaceuticals (United States)


Published in SPIE Proceedings Vol. 3515:
Microfluidic Devices and Systems
A. Bruno Frazier; Chong Hyuk Ahn, Editor(s)

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