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Proceedings Paper

Fluorescence spectroscopy of bacteriorhodopsin at room temperature
Author(s): Atanaska Andreeva; Villen Kolev; Tzvetana Lazarova
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Paper Abstract

The chromophore fluorescence of bacteriorhodopsin (BR) has been studied by using visible lines of an argon and He-Ne laser at room temperature. This fluorescence is extremely weak and appears as an unstructured band in the 650 - 880 nm range. The same line shape of the emission spectra of dark- and light-adapted BR recorded at low light excitation intensity implies that the emission of 13-cis conformation of retinal is not observable at these conditions. The analysis of the spectra recorded at different pH, intensity and wavelength of excitation light show that the spectrum of BR in fact contains at least three emission bands. They can be assigned to all-trans BR, O and Q intermediates, accumulated during the photocycle. As a most probable emission due to only all-trans BR in light-adapted membrane, we assign a band with a maximum located at 730 nm (13700 cm-1) and a full width of half maximum equals 2580 cm-1.

Paper Details

Date Published: 19 August 1998
PDF: 4 pages
Proc. SPIE 3573, OPTIKA '98: 5th Congress on Modern Optics, (19 August 1998); doi: 10.1117/12.320967
Show Author Affiliations
Atanaska Andreeva, Sofia Univ. (Bulgaria)
Villen Kolev, Institute of Biophysics (Bulgaria)
Tzvetana Lazarova, Institute of Biophysics (Bulgaria)

Published in SPIE Proceedings Vol. 3573:
OPTIKA '98: 5th Congress on Modern Optics
Gyorgy Akos; Gabor Lupkovics; Andras Podmaniczky, Editor(s)

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