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Proceedings Paper

Laser SERs spectrum of GLP in Ag sol
Author(s): PeiDi Bao; Lang Bao; TianQuan Huang; XinMing Liu
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Paper Abstract

We have obtained and investigated the laser Surface-Enhanced Raman Scattering (SERS) spectrum of Leptospiral Glycolipoproteim (GLP). The SERS spectrum of GLP offers the structural information of the molecules and provides the evidence that the functionality of the molecules are preserved on the Ag surface. The SERS spectrum and its Ordinary Raman Scattering (ORS) spectrum of GLP show their correlations. In the ORS spectrum, the intense AmI at 1651 cm-1 and weak AmIII at 1283 cm-1 show that GLP has a predominantly (alpha) -helix secondary structure. The Raman line at 542 cm-1 indicates that the molecules of GLP has trans-gauch-trans configuration of the C-C-S-S-C-C linkage. In the SERS spectrum, the low-frequency band at 239 cm-1 is attributed to the metal-molecule vibration, indicating chemisorption of the molecules of GLP on Ag surface. The bands belong to (delta) (COO-) at 620, 683 cm-1, the band belongs to (upsilon) s(C-COO-) at 921 cm-1, belongs to (upsilon) (C-N), (upsilon) s(COO-) and (delta) (NH2) at 1158, 1366 and 1648 cm-1. It suggests that the molecules of GLP were adsorbed on Ag surface through both carboxylate groups and amino groups. The Raman enhancement of GLP has a short-range mechanism. The laser SERS spectrum enhancement factor of GLP is about 3 X 102.

Paper Details

Date Published: 7 August 1998
PDF: 5 pages
Proc. SPIE 3548, Biomedical Optics and Lasers: Diagnostics and Treatment, (7 August 1998); doi: 10.1117/12.317869
Show Author Affiliations
PeiDi Bao, Sichuan Univ. (China)
Lang Bao, Univ. of Medical Science (China)
TianQuan Huang, Sichuan Univ. (China)
XinMing Liu, Sichuan Univ. (China)


Published in SPIE Proceedings Vol. 3548:
Biomedical Optics and Lasers: Diagnostics and Treatment
Junheng Li; James A. Harrington, Editor(s)

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