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Proceedings Paper

Three-dimensional reconstruction of the cell microscopic image
Author(s): Luguo Hao; Ming Liu; Shouping Nie; SongLing Bian; ChunKan Tao; Wanrong Gao; Ge Kou
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Paper Abstract

At the optical processing domain, the three-dimension reconstruction of information is an important problem of research. At the last few years, the laser confocal scanning microscope (LCSM) system was researched and has been used successfully as advanced optical instrument in the biological and medical research range. This paper primarily research the 3-D image reconstruction of the cell using its 2-D section image formed by the laser confocal scanning microscopy. First, the paper rests on the LCSM system characteristic of forming image to analyze the image noise and remove it. Then we extract the information of 2-D section image edge, and we use these informations to reconstruct the 3-D image of cell surface by method of B-Spline.

Paper Details

Date Published: 7 August 1998
PDF: 6 pages
Proc. SPIE 3548, Biomedical Optics and Lasers: Diagnostics and Treatment, (7 August 1998); doi: 10.1117/12.317857
Show Author Affiliations
Luguo Hao, Nanjing Univ. of Science and Technology (China)
Ming Liu, Nanjing Univ. of Science and Technology (China)
Shouping Nie, Nanjing Univ. of Science and Technology (China)
SongLing Bian, Nanjing Univ. of Science and Technology (China)
ChunKan Tao, Nanjing Univ. of Science and Technology (China)
Wanrong Gao, Nanjing Univ. of Science and Technology (China)
Ge Kou, Nanjing Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 3548:
Biomedical Optics and Lasers: Diagnostics and Treatment
Junheng Li; James A. Harrington, Editor(s)

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