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Proceedings Paper

UV laser radiosensitivity of normal and tumoral chromatin
Author(s): Liliana Radu; Ion N. Mihailescu; V. Preoteasa; Irina Radulescu; Doina Gostian; Alexandru Hening; S. Radu
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Paper Abstract

A normal and a tumoral chromatin radiosensitivity to an UV laser radiation was determined. The characteristics of these chromatin samples were established by the analysis of the absorption and emission spectra of chromatin complexes with a specific DNA ligand-ethidium bromide, by Scatchard representations of ligand binding to chromatin and by the Forster energy transfer efficiency determination between two fluorescent ligands coupled at chromatin: dansyl chloride and acridine orange. The effects of excimer laser beam with (lambda) equals 248 nm on chromatin structure were analyzed by the above methods and also by the establishment of the intrinsic chromatin fluorescence and of the excited state lifetimes of a specific DNA ligand.

Paper Details

Date Published: 2 July 1998
PDF: 8 pages
Proc. SPIE 3405, ROMOPTO '97: Fifth Conference on Optics, (2 July 1998); doi: 10.1117/12.312827
Show Author Affiliations
Liliana Radu, Victor Babes Institute (Romania)
Ion N. Mihailescu, National Institute for Laser, Plasma, and Radiation Physics (Romania)
V. Preoteasa, Victor Babes Institute (Romania)
Irina Radulescu, Victor Babes Institute (Romania)
Doina Gostian, Victor Babes Institute (Romania)
Alexandru Hening, National Institute for Laser, Plasma, and Radiation Physics (Romania)
S. Radu, Polytehnica Univ. of Bucharest (Romania)


Published in SPIE Proceedings Vol. 3405:
ROMOPTO '97: Fifth Conference on Optics
Valentin I. Vlad; Dan C. Dumitras, Editor(s)

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