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Proceedings Paper

Comparison of the effect of UV laser radiation and of a radiomimetic substance on chromatin
Author(s): Irina Radulescu; Liliana Radu; Ruxandra Serbanescu; V. D. Nelea; C. Martin; Ion N. Mihailescu
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Paper Abstract

The damages of the complex of deoxyribonucleic acid (DNA) and proteins from chromatin, produced by the UV laser radiation and/or by treatment with a radiomimetic substance, bleomycin, were compared. The laser radiation and bleomycin effects on chromatin structure were determined by the static and dynamic fluorimetry of chromatin complexes with the DNA specific ligand-- proflavine and by the analysis of tryptophan chromatin intrinsic fluorescence. Time resolved spectroscopy is a sensitive technique which allows to determine the excited state lifetimes of chromatin--proflavine complexes. Also, the percentage contributions to the fluorescence of proflavine, bound and unbound to chromatin DNA, were evaluated. The damages produced by the UV laser radiation on chromatin are similar with those of radiomimetic substance action and consists in DNA and proteins destruction. The DNA damage degree has been determined. The obtained results may constitute some indications in the laser utilization in radiochimiotherapy.

Paper Details

Date Published: 2 July 1998
PDF: 5 pages
Proc. SPIE 3405, ROMOPTO '97: Fifth Conference on Optics, (2 July 1998); doi: 10.1117/12.312650
Show Author Affiliations
Irina Radulescu, Victor Babes Institute (Romania)
Liliana Radu, Victor Babes Institute (Romania)
Ruxandra Serbanescu, Univ. of Bucharest (Romania)
V. D. Nelea, National Institute for Laser, Plasma, and Radiation Physics (Romania)
C. Martin, National Institute for Laser, Plasma, and Radiation Physics (Romania)
Ion N. Mihailescu, National Institute for Laser, Plasma, and Radiation Physics (Romania)

Published in SPIE Proceedings Vol. 3405:
ROMOPTO '97: Fifth Conference on Optics
Valentin I. Vlad; Dan C. Dumitras, Editor(s)

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