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Proceedings Paper

Comparison of photonic and electromagnetic effects on the human leukocyte
Author(s): Michael A. DellaVecchia; Richard B. Beard; D. Feng; Xiaoyan Dai; Kambiz Pourrezaei; Alexander V. Priezzhev
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Paper Abstract

The dielectric and magnetic influence on human cells have been widely studied previously by the authors. Recently, the effects of energy in the visible electromagnetic spectrum have been investigated. In this subsequent study, the photonic effects on the in vitro migration of the polymorphonuclear and mononuclear leukocytes are compared with the corresponding electromagnetic field effects. Dielectric spectra of the polymorph in the 300 KHz to 400 KHz and 700 KHz to 800 KHz range have been measured. At frequencies of 350 KHz and 720 KHz an increase in the migration of the polymorphonuclear leukocyte have been observed. This stimulation was attributed to the charges on the nuclear surface. Recent preliminary data have shown a similar increased migration in the 20 MHz range. Photonic studies have indicated an enhanced migration for the polymorphonuclear leukocytes at a wavelength of 660 nm (red) and an inhibited migration at 565 nm (green). The photonic effects were postulated to be the results of a biochemical interaction rather than a membranous surface charge displacement secondary to an electric field. The migration of the white blood cells were measurement via the Boyden chamber technique and expressed in terms of a cytokinetic index which expresses the cellular movement independent of its environmental concentration gradient.

Paper Details

Date Published: 19 June 1998
PDF: 9 pages
Proc. SPIE 3252, Optical Diagnostics of Biological Fluids III, (19 June 1998); doi: 10.1117/12.311894
Show Author Affiliations
Michael A. DellaVecchia, Will Eye Hospital and Drexel Univ. (United States)
Richard B. Beard, Will Eye Hospital and Drexel Univ. (United States)
D. Feng, Will Eye Hospital and Drexel Univ. (United States)
Xiaoyan Dai, Will Eye Hospital and Drexel Univ. (United States)
Kambiz Pourrezaei, Drexel Univ. (United States)
Alexander V. Priezzhev, Moscow State Univ. (Russia)


Published in SPIE Proceedings Vol. 3252:
Optical Diagnostics of Biological Fluids III
Alexander V. Priezzhev; Toshimitsu Asakura; J. David Briers, Editor(s)

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