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Proceedings Paper

Noninvasive fluorescence detection of physiological function
Author(s): Richard B. Dorshow; Joseph E. Bugaj
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Paper Abstract

A non-invasive in vivo fluorescence detection scheme was employed to continuously monitor exogenous dye clearance from the vasculature. Differentiation between normal and abnormal organ function in a rat model was demonstrated for both liver and kidney. A fiber optic transmitted excitation light from source to ear; a second fiber optic positioned near the ear transmitted the emitted fluorescent light to a detector system. Several dyes were employed in the initial feasibility studies. Indocyanine green, known to be exclusively cleared from the blood stream by the liver, was excited in vivo with laser light at 780 nm. The fluorescence signal was detected at 830 nm. Characteristic clearance curves of normal hepatic function were obtained, with decay rates that agreed well with literature values. After surgically performing a partial hepatectomy, clearance curves with greatly reduced decay rates were obtained as expected. Additionally, fluorescein labeled polymers and peptides were excited in vivo with laser light at 488 nm. The fluorescence signal was detected at 520 nm. Characteristic clearance curves of normal renal function were subsequently obtained. Thus, the feasibility of a new non- invasive method for physiological function assessment was established.

Paper Details

Date Published: 19 June 1998
PDF: 7 pages
Proc. SPIE 3252, Optical Diagnostics of Biological Fluids III, (19 June 1998); doi: 10.1117/12.311887
Show Author Affiliations
Richard B. Dorshow, Mallinckrodt Inc. (United States)
Joseph E. Bugaj, Mallinckrodt Inc. (United States)


Published in SPIE Proceedings Vol. 3252:
Optical Diagnostics of Biological Fluids III
Alexander V. Priezzhev; Toshimitsu Asakura; J. David Briers, Editor(s)

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