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Proceedings Paper

Hyperspectral Raman imaging of bone growth and regrowth chemistry
Author(s): Jerilyn A. Pezzuti; Michael D. Morris; Jeffrey F. Bonadio; Steven A. Goldstein
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Paper Abstract

Hyperspectral Raman microscopic imaging of carbonated hydroxyapatite (HAP) is used to follow the chemistry of bone growth and regrowth. Deep red excitation is employed to minimize protein fluorescence interference. A passive line generator based on Powell lens optics and a motorized translation stage provide the imaging capabilities. Raman image contrast is generated from several lines of the HAP Raman spectrum, primarily the PO4-3. Factor analysis is used to minimize the integration time needed for acceptable contrast and to explore the chemical species within the bone. Bone age is visualized as variations in image intensity. High definition, high resolution images of newly formed bone and mature bone are compared qualitatively. The technique is currently under evaluation for study of experimental therapies for fracture repair.

Paper Details

Date Published: 9 June 1998
PDF: 7 pages
Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); doi: 10.1117/12.310566
Show Author Affiliations
Jerilyn A. Pezzuti, Univ. of Michigan (United States)
Michael D. Morris, Univ. of Michigan (United States)
Jeffrey F. Bonadio, Univ. of Michigan Medical School (United States)
Steven A. Goldstein, Univ. of Michigan Medical School (United States)


Published in SPIE Proceedings Vol. 3261:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V
Thomas Taiwei Lu; Carol J. Cogswell; Jeremy M. Lerner; Jose-Angel Conchello; Jeremy M. Lerner; Thomas Taiwei Lu; Tony Wilson, Editor(s)

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