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Proceedings Paper

Tracking on treelike structures in 3D confocal images
Author(s): Andreas Herzog; Gerald Krell; Bernd Michaelis; Werner Zuschratter
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Paper Abstract

Confocal microscopy is qualified to perform volume scans of nerve cells with dendrites and spines. Length and diameter of the dendrite branches and the spines should be determined to analyze the influence of learning processes. A prerequisite for that is the recognition of the dendritic structure with branching off and spines. Because the microscope operates at the resolution limit the images are blurry, noisy and only poorly sampled. In contrast to other methods which are based on binary images and thinning algorithms, our method tracks and dendritic tree faster and in the gray-level domain using simple geometric models.An explicit segmentation is unnecessary and knowledge about shape and structure of the dendrite is included as a priori information. For large trees, first a low resolution scan is captured to crete a rough model. The algorithm allows to refine this model using higher resolution scans for interesting regions along the dendrite. The large unimportant areas between the dendrite branches are not scanned at high resolution to save time and disc space. In a second step, the parameters of the model are adapted to the microscope image by minim zing the deviation of the microscope image from the mode image convolved by the microscope point spread function. Features like number, diameter, length and position of the dendrite branches and spines can be easily calculated from the model. An interactive user intervention is possible at the model domain.

Paper Details

Date Published: 9 June 1998
PDF: 12 pages
Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); doi: 10.1117/12.310550
Show Author Affiliations
Andreas Herzog, Otto-von-Guericke-Univ. Magdeburg (Germany)
Gerald Krell, Otto-von-Guericke-Univ. Magdeburg (Germany)
Bernd Michaelis, Otto-von-Guericke-Univ. Magdeburg (Germany)
Werner Zuschratter, Federal Institute for Neurobiology (Germany)

Published in SPIE Proceedings Vol. 3261:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V
Thomas Taiwei Lu; Carol J. Cogswell; Jeremy M. Lerner; Jose-Angel Conchello; Jeremy M. Lerner; Thomas Taiwei Lu; Tony Wilson, Editor(s)

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