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Proceedings Paper

Three-dimensional limited-angle microtomography of blood cells: experimental results
Author(s): Gennady G. Levin; Gennady N. Vishnyakov; Constantin S. Zakarian; Alexey V. Likhachov; Valery Vladimirov Pickalov; Gennady I. Kozinets; Julia K. Novoderzhkina; Elena A. Streletskaya
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Paper Abstract

Tomographic measurements of the 3D refractive index spatial distribution within optically transparent phase samples with computerized interferometric microscopes are proposed. Phase shifting interferometric microtomography applications for the 3D image reconstruction of the blood cells are represented. The immersion 100x, N.A. equals 1.25 objective was used to increase the spatial resolution and observation angle range to 90 degree. ART, combined ART and iterative Gerchberg-Papoulis 3D algorithm were used for the tomogram reconstruction. To determine the accuracy and spatial resolution of the blood cells image reconstruction by means of the interferometric microtomographic method the numerical simulations were implemented.

Paper Details

Date Published: 9 June 1998
PDF: 6 pages
Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); doi: 10.1117/12.310549
Show Author Affiliations
Gennady G. Levin, Russian Research Institute for Optophysical Measurements (Russia)
Gennady N. Vishnyakov, Russian Research Institute for Optophysical Measurements (Russia)
Constantin S. Zakarian, Russian Research Institute for Optophysical Measurements (Russia)
Alexey V. Likhachov, Institute of Theoretical and Applied Mechanics (Russia)
Valery Vladimirov Pickalov, Institute of Theoretical and Applied Mechanics (Russia)
Gennady I. Kozinets, Hematology Scientific Ctr./Russian Academy of Medical Science (Russia)
Julia K. Novoderzhkina, Hematology Scientific Ctr./Russian Academy of Medical Science (Russia)
Elena A. Streletskaya, Hematology Scientific Ctr./Russian Academy of Medical Science (Russia)


Published in SPIE Proceedings Vol. 3261:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V
Thomas Taiwei Lu; Carol J. Cogswell; Jeremy M. Lerner; Jose-Angel Conchello; Jeremy M. Lerner; Thomas Taiwei Lu; Tony Wilson, Editor(s)

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