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Proceedings Paper

Two-dimensional defocusing correction using artificial neural nets
Author(s): Carlos Ortiz de Solorzano; Vicente Gonzalez; Andres Santos; Francisco del Pozo
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Paper Abstract

The aim of this work is to show that properly trained ANNs can be used as an image restoration tool to correct the effect of defocusing on 2D optical microscopy images. The proposed method can be applied to correct the results of inaccurate range focusing algorithms on fully automated imaging and analysis systems and to compensate the effect of the limited of the depth of focusing on high numerical aperture system. One type of ANN was used: feedforward multilayer perceptron, with supervised back propagation training. Its performance has been tested with both synthetic images and real images from latex microspheres. The network was trained with sets of pairs of images: each pair consisted of a defocused image and its corresponding in-focus version. Different levels of defocusing were used. The criteria used to select the algorithm parameters to tune the networks and to train them will be presented. The results of the experiments performed to test their ability to 'learn' to correct the defocusing and to generalize the results will also be shown. The result show that, when trained with images with some levels of defocusing, the network was able to learn and accurately correct these defocusing levels, but it can also generalize the results and correct other levels of defocusing.

Paper Details

Date Published: 9 June 1998
PDF: 12 pages
Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); doi: 10.1117/12.310546
Show Author Affiliations
Carlos Ortiz de Solorzano, Lawrence Berkeley National Lab. and Univ. Politecnica de Madrid (United States)
Vicente Gonzalez, Univ. Politecnica de Madrid (Spain)
Andres Santos, Univ. Politecnica de Madrid (Spain)
Francisco del Pozo, Univ. Politecnica de Madrid (Spain)

Published in SPIE Proceedings Vol. 3261:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V
Thomas Taiwei Lu; Carol J. Cogswell; Jeremy M. Lerner; Jose-Angel Conchello; Jeremy M. Lerner; Thomas Taiwei Lu; Tony Wilson, Editor(s)

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