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Proceedings Paper

Comparing methods for 3D microscopy
Author(s): James G. McNally; Joanne Markham; Jose-Angel Conchello
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Paper Abstract

In previous studies, we examined 3D images of a latex bead containing a surface layer of fluorescence whose thickness was determined by physical sectioning. Confocal images of the bead from six different microscopes all exhibited a significantly thicker fluorescence shell than actually present. In contrast, deconvolved wide-field images of the bead produced an image with the correct shell thickness. We have now repeated some of these studies using a new latex bed containing a much thinner layer of surface fluorescence. In contrast to earlier studies, confocal image of this bead appear to show an accurate thickness for the fluorescence shell. These particular confocal microscopes also yielded essentially aberration free images, which was not the case for some of the earlier microscopes tested. In these recent studies however, deconvolution of the bead appeared less robust than earlier. Of three independent wide-field images of the bead, only one yielded a substantially artifact-free image upon deconvolution.

Paper Details

Date Published: 9 June 1998
PDF: 9 pages
Proc. SPIE 3261, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V, (9 June 1998); doi: 10.1117/12.310541
Show Author Affiliations
James G. McNally, Institute for Biomedical Computing/Washington Univ. (United States)
Joanne Markham, Institute for Biomedical Computing/Washington Univ. (United States)
Jose-Angel Conchello, Institute for Biomedical Computing/Washington Univ. (United States)


Published in SPIE Proceedings Vol. 3261:
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing V
Thomas Taiwei Lu; Carol J. Cogswell; Jeremy M. Lerner; Jose-Angel Conchello; Jeremy M. Lerner; Thomas Taiwei Lu; Tony Wilson, Editor(s)

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