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Proceedings Paper

New method for measurement of in-vivo penetration of UVR into the crystalline lens
Author(s): Per G. Soederberg; Stefan Loefgren; Ralph Michael; Xochitl Gonzales-Cirre
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Paper Abstract

The penetration of 300 nm ultraviolet radiation (UVR) in the anterior cortex of the crystalline lens was 0.45 mm, penetration being expressed as the distance attenuating the transmittance to 1/e2. The estimation is based on measurement of in vivo inactivation of lactate dehydrogenase (LDH) due to in vivo exposure to UVR 300 nm. The inactivation of LDH was independent of the latency interval after the exposure within 0 - 6 h. There was a 30 - 40% inactivation of LDH in the cornea that dropped to 0% in the inner anterior cortex. The currently described method allows spatially resolved measurement of the in vivo dose of UVR 300 nm within the cornea and the crystalline lens.

Paper Details

Date Published: 1 June 1998
PDF: 5 pages
Proc. SPIE 3246, Ophthalmic Technologies VIII, (1 June 1998); doi: 10.1117/12.309434
Show Author Affiliations
Per G. Soederberg, St. Eriks Eye Hospital (Sweden)
Stefan Loefgren, St. Eriks Eye Hospital (Sweden)
Ralph Michael, St. Eriks Eye Hospital (Netherlands)
Xochitl Gonzales-Cirre, St. Eriks Eye Hospital (Sweden)


Published in SPIE Proceedings Vol. 3246:
Ophthalmic Technologies VIII
Pascal O. Rol; Karen Margaret Joos; Fabrice Manns, Editor(s)

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