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Proceedings Paper

Sensitive on-column absorbance detection of native molecules
Author(s): Sandrine Berniolles; Hongjing Kan; Lu Dai; James E. Knittle; William M. Tong
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Paper Abstract

Laser wave mixing is presented as a sensitive detection method for absorbance measurements in flowing liquid analytes. Wave mixing is an unusually sensitive multi-photon nonlinear optical method since the analytical signal is generated as a coherent laser beam. Since the bright signal is visible to the naked eye, optical alignment is convenient. For liquid analytes in continuously flowing cells, we have demonstrated excellent detection sensitivity levels using various wave-mixing optical configurations and laser sources. Since it is an optical absorption method, laser wave-mixing detection offers excellent detection sensitivity for both fluorescing and non-fluorescing analytes. Hence, one does not have to label non-fluorescing analytes with tags in order to obtain good detection sensitivity in wave-mixing detection methods. Sensitivity detection of analytes in their native form offers many obvious advantages especially when interfaced to popular capillary separation methods. Since the analyte laser probe volume is very small, wave-mixing detection is suitable for on-column detection in various capillary electrophoresis or micro liquid chromatography systems.

Paper Details

Date Published: 15 May 1998
PDF: 7 pages
Proc. SPIE 3270, Methods for Ultrasensitive Detection, (15 May 1998); doi: 10.1117/12.308380
Show Author Affiliations
Sandrine Berniolles, San Diego State Univ. (United States)
Hongjing Kan, San Diego State Univ. (United States)
Lu Dai, San Diego State Univ. (United States)
James E. Knittle, San Diego State Univ. (United States)
William M. Tong, San Diego State Univ. (United States)


Published in SPIE Proceedings Vol. 3270:
Methods for Ultrasensitive Detection
Bryan L. Fearey, Editor(s)

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