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Proceedings Paper

Effect of laser irradiation on the functional activity of enzymes with different structural complexity
Author(s): Svetlana A. Ostrovtsova; Alexander P. Volodenkov; Alexander A. Maskevich; Irina M. Artsukevich; Slavomir S. Anufrik; Alexander F. Makarchikov; Ivan P. Chernikevich; Vitali I. Stepuro
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Paper Abstract

Three enzymes differing in their structural composition were irradiated by UV lasers to study the effect of temperature, protein concentration and addition of small molecules on their sensitivity to radiation exposure. The laser-induced effects were due to the structural complexity of the protein molecules and depended on the dose applied, the wavelength and the density of irradiation. The multi-enzyme 2- oxoglutarate dehydrogenase complex was subjected to pronounced irradiation-induced changes whereas the response of the two other enzymes was less significant. Reduction of the protein levels in irradiated samples was important under the XeCl laser coercion and the effects depended on the doses applied. The laser irradiation effects are suggested to be realized by means of conformational changes in the protein molecules and intermolecular association- dissociation processes.

Paper Details

Date Published: 18 May 1998
PDF: 7 pages
Proc. SPIE 3255, Applications of Ultrashort-Pulse Lasers in Medicine and Biology, (18 May 1998); doi: 10.1117/12.308213
Show Author Affiliations
Svetlana A. Ostrovtsova, Institute of Biochemistry (Belarus)
Alexander P. Volodenkov, State Univ. of Grodno (Belarus)
Alexander A. Maskevich, State Univ. of Grodno (Belarus)
Irina M. Artsukevich, Institute of Biochemistry (Belarus)
Slavomir S. Anufrik, State Univ. of Grodno (Belarus)
Alexander F. Makarchikov, Institute of Biochemistry (Belarus)
Ivan P. Chernikevich, Institute of Biochemistry (Belarus)
Vitali I. Stepuro, State Univ. of Grodno (Belarus)

Published in SPIE Proceedings Vol. 3255:
Applications of Ultrashort-Pulse Lasers in Medicine and Biology
Joseph Neev, Editor(s)

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