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Proceedings Paper

Resolution degradation due to brain tissue scattering
Author(s): Chun-Ming Albert Wang; J. Harold Wayland; Scott E. Fraser
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Paper Abstract

We have investigated the imaging of structural details at various depths within rat brain tissue to better understand the loss of resolution and contrast due in large part to light scattering. A target was imaged through various thicknesses of tissue; the normalized slope of transition between light and dark regions of the images was used as an effective resolution index. The ERI improves dramatically as the wavelength is increased: 0.03 at 600nm, 0.3 at 850nm for a 270 micrometers -thick hippocampus slice. A comparable change was noted for a 270 micrometers -thick cortex slice. For a fixed wavelength, ERI decreases for thicker hippocampus slices, form 0.67 at 220 micrometers to 0.31 at 250 micrometers and to 0.24 at 300 micrometers . Image contrast improves with longer wavelength: from 0.9 at 600nm to 9.5 at 850 nm; from 0.9 at 600nm to 4.6 at 850nm. Despite regional differences in the transparent of brain regions, images were degraded less by scattering at longer wavelengths, arguing strongly for the use of near-IR wavelengths for microscopic imaging either with transmitted light or fluorescent emission for exploring deeper biological structures.

Paper Details

Date Published: 1 May 1998
PDF: 11 pages
Proc. SPIE 3253, Biomedical Sensing and Imaging Technologies, (1 May 1998); doi: 10.1117/12.308018
Show Author Affiliations
Chun-Ming Albert Wang, California Institute of Technology (Taiwan)
J. Harold Wayland, California Institute of Technology (United States)
Scott E. Fraser, California Institute of Technology (United States)

Published in SPIE Proceedings Vol. 3253:
Biomedical Sensing and Imaging Technologies
Robert A. Lieberman; Tuan Vo-Dinh, Editor(s)

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