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Proceedings Paper

Comparison between time-correlated single-photon counting and fluorescence correlation spectroscopy in single-molecule identification
Author(s): Joerg Enderlein; Malte Koellner
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Paper Abstract

Currently, two methods of detection and identification of single molecules are widely used: fluorescence correlation spectroscopy (FCS) and time-correlated single photon counting (TCSPC). We present a thorough theoretical analysis of the error rates for identifying single molecules according to their diffusion coefficients (using FCS), and to their fluorescence lifetimes (using TCSPC). In most cases, the error rate using TCSPC is much lower. TCSPC is thus proven to be more versatile for analyzing single molecule events. The study is significant for a broad range of ultrasensitive fluorescence detection applications.

Paper Details

Date Published: 10 April 1998
PDF: 10 pages
Proc. SPIE 3259, Systems and Technologies for Clinical Diagnostics and Drug Discovery, (10 April 1998); doi: 10.1117/12.307340
Show Author Affiliations
Joerg Enderlein, Univ. Regensburg (Germany)
Malte Koellner, Patentanwaelte Zenz, Helber, Hosbach & Pa. (Germany)


Published in SPIE Proceedings Vol. 3259:
Systems and Technologies for Clinical Diagnostics and Drug Discovery
Gerald E. Cohn; John C. Owicki, Editor(s)

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