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Proceedings Paper

Near-field scanning optical microscope with an optically trapped metallic Rayleigh particle
Author(s): Tadao Sugiura; Takao Okada
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Paper Abstract

We developed a near-field scanning optical microscope (NSOM) which uses an optically trapped metallic Rayleigh particle as a near-field probe. This microscope is suitable for observations of biological specimen under aqueous condition because the probe particle is held by non-contact force from radiation pressure. The spring constant of trapping force is so weak (several mN/m order typically) that induced damage on a sample rarely occurs by this force. We used a gold colloidal particle which had a diameter of 40 nm as a probe. The scattering efficiency of the particle (at 488-nm wavelength, nAu equals 0.916, KAu equals 1.840) is 348 times as great as that of a glass particle (nglass equals 1.5) in the same size under water. We investigated a surface of a cover glass with the gold colloidal particle and confirmed the ability to observe small irregularities of the surface in around 10 nm. We also observed aggregated gold colloidal particles (d equals 40 nm) on a cover glass and fluorescent beads on glass. The investigation of DNA stained with YOYO-1 iodide on glass under water conditions was also performed.

Paper Details

Date Published: 29 April 1998
PDF: 11 pages
Proc. SPIE 3260, Optical Investigations of Cells In Vitro and In Vivo, (29 April 1998); doi: 10.1117/12.307080
Show Author Affiliations
Tadao Sugiura, Joint Research Ctr. for Atom Technology (Japan)
Takao Okada, Joint Research Ctr. for Atom Technology (Japan)


Published in SPIE Proceedings Vol. 3260:
Optical Investigations of Cells In Vitro and In Vivo
Robert C. Leif; Daniel L. Farkas; Robert C. Leif; Bruce J. Tromberg, Editor(s)

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