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Proceedings Paper

Fluorescence anisotropy controlled by light quenching
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Paper Abstract

We demonstrated that fluorescence anisotropy can be effectively decreased or increased in the presence of light quenching, depending on relative polarizations of excitation and quenching pulses. For parallel light quenching anisotropy decreases to 0.103 and z-axis symmetry is being preserved. In the presence of perpendicular light quenching, the steady- state anisotropy of pyridine 2 glycerol solution increases from 0.368 for unquenched sample to 0.484, for quenched one. We show that angular distribution of transition moments loses the z-axis symmetry in the presence of perpendicular light quenching. In these cases we used more general definitions of anisotropy. Induced by light quenching anisotropy can be applied in both, steady-state and time-resolved measurements. In particular, the systems with low or none anisotropy can be investigated with proposed technique.

Paper Details

Date Published: 1 May 1998
PDF: 11 pages
Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); doi: 10.1117/12.307076
Show Author Affiliations
Ignacy Gryczynski, Univ. of Maryland School of Medicine/Baltimore (United States)
Zygmunt Gryczynski, Univ. of Maryland School of Medicine/Baltimore (United States)
Joseph R. Lakowicz, Univ. of Maryland School of Medicine/Baltimore (United States)


Published in SPIE Proceedings Vol. 3256:
Advances in Optical Biophysics
Joseph R. Lakowicz; J. B. Alexander Ross, Editor(s)

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