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Proceedings Paper

Applications of surface-enhanced spectroscopic techniques to biosystems
Author(s): Gerald Steiner; Christian Kuhne; Beate Leupolt; Wolfgang B. Fischer; Reiner Salzer
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Paper Abstract

The potential of surface enhanced spectroscopy consists in the detection of very small but specific structures of biomaterials. Surface enhanced infrared absorption (SEIRA) yields fingerprint information on the biomaterials. Electronic interactions between individual groups in the molecule are detected by means of surface enhanced fluorescence (SEF). During the investigation of biomembranes with SEIRA a tenfold intensity enhancement could be obtained. Enhancement factors greater than 100 can be achieved with SEF. Enhancement is considerably influenced by the properties of the metal cluster structure. Biomembranes formed from vesicles containing the nicotinic acetylcholine receptor were spectroscopically characterized. The adsorption of the vesicles on Ge- and Ag surfaces was investigated. The metal cluster structure was optimized in order to obtain high intensity enhancement factors.

Paper Details

Date Published: 1 May 1998
PDF: 7 pages
Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); doi: 10.1117/12.307072
Show Author Affiliations
Gerald Steiner, Technische Univ. Dresden (Germany)
Christian Kuhne, Technische Univ. Dresden (Germany)
Beate Leupolt, Technische Univ. Dresden (Germany)
Wolfgang B. Fischer, Technische Univ. Dresden (Germany)
Reiner Salzer, Technische Univ. Dresden (Germany)

Published in SPIE Proceedings Vol. 3256:
Advances in Optical Biophysics
Joseph R. Lakowicz; J. B. Alexander Ross, Editor(s)

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