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Proceedings Paper

Differential two-color x-ray radiobiology of membrane/cytoplasm in yeast cells and lymphocytes
Author(s): Marziale Milani; Monica Ballerini; Giuliana Baroni; Dimitri Batani; Simona Cozzi; Lorenzo Ferraro; Michele Costato; Francesca Salsi; Achille Pozzi; Franco Squadrini; I. C. Edmond Turcu; Ric M. Allott; Nicola Lisi; Waseem Shaikh; Shaun Hughes
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Paper Abstract

An overall approach into the differential investigation of membrane/cytoplasm related metabolism and of cell-cycle of yeast cells after two color soft X-ray irradiation is presented; the soft X-rays being generated in trains of picosecond pulses by laser-plasma interaction. The two color X-ray differential technique is based on the generation of approximately 0.6 KeV X-rays which are deposited only in the membrane-wall complex switching off the anaerobic (fermentative) activity of yeast cells and on the generation of approximately 1.2 KeV X-rays which are mainly deposited in the cytoplasm, mitochondria and nucleus of yeast cells, mainly affecting the aerobic metabolism. A synergetic analysis of the metabolism is discussed, mainly founded on the recording of different correlated metabolic parameters, both on-line and delayed. Among the relevant access, pressure monitoring in batch samples acquire a dominant role allowing the identification of metabolic oscillation, that represent a marker of physical and chemical actions performed on the samples at different times. The experience acquired on yeast cells metabolism is being used to investigate lymphocytes metabolism and the related oscillatory properties of relevant enzymatic complexes. Actually even if it is not exactly the same as the mammalian situation, it should really propel the whole field forward.

Paper Details

Date Published: 1 May 1998
PDF: 11 pages
Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); doi: 10.1117/12.307071
Show Author Affiliations
Marziale Milani, Univ. of Milano (Italy) and INFM (Italy)
Monica Ballerini, Univ. of Milano (Italy)
Giuliana Baroni, Univ. of Milano (Italy)
Dimitri Batani, Univ. of Milano (Italy) and INFM (Italy)
Simona Cozzi, Univ. of Milano (Italy)
Lorenzo Ferraro, Univ. of Milano (Italy)
Michele Costato, Univ. of Modena (Italy)
Francesca Salsi, Univ. of Modena (Italy)
Achille Pozzi, Univ. of Modena (Italy)
Franco Squadrini, Univ. of Modena (Italy)
I. C. Edmond Turcu, Rutherford Appleton Lab. (United States)
Ric M. Allott, Rutherford Appleton Lab. (United Kingdom)
Nicola Lisi, Rutherford Appleton Lab. (United Kingdom)
Waseem Shaikh, Rutherford Appleton Lab. (United Kingdom)
Shaun Hughes, Rutherford Appleton Lab. (United Kingdom)

Published in SPIE Proceedings Vol. 3256:
Advances in Optical Biophysics
Joseph R. Lakowicz; J. B. Alexander Ross, Editor(s)

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