Share Email Print
cover

Proceedings Paper

Long-lived highly luminescent rhenium (I) metal-ligand complex as a probe of biomolecules
Author(s): Felix N. Castellano; Xiang-Qun Guo; Li Li; Henryk Szmacinski; Jeffrey Sipior; Joseph R. Lakowicz
Format Member Price Non-Member Price
PDF $14.40 $18.00

Paper Abstract

A luminescent rhenium(I) metal-ligand complex, [Re(bcp)(CO)3(4-COOHPy)](ClO4), where bcp is 2,9-dimethyl-4,7-diphenyl-1,10-phenanthroline and 4-COOHPy is isonicotinic acid, has been synthesized and characterized. This complex displays high quantum yields and long excited- state lifetimes (0.3 - 10 microseconds) in fluid solution at room temperature. The metal-to-ligand charge transfer (MLCT) emission from this compound exhibits sensitivity to micro- environment. [Re(bcp)(CO)3(4-COOHPy)]+ displays highly polarized emission with a maximum anisotropy near 0.3 in the absence of rotational diffusion. This Re(I) MLCT complex was conjugated to several biomolecules, including the proteins human serum albumin (HSA) and bovine immunoglobulin G(IgG) as well as an amine containing lipid. When bound to a protein or lipid, the decay time is near 3 microseconds and the quantum yield is approximately 0.12 in aqueous air-equilibrated solution at room temperature. The unique spectral properties and reactive carboxylic acid functionality of [Re(bcp)(CO)3(4-COOHPy)]+, allowed utilization of this probe in numerous biophysical and biomedical applications.

Paper Details

Date Published: 1 May 1998
PDF: 11 pages
Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); doi: 10.1117/12.307064
Show Author Affiliations
Felix N. Castellano, Univ. of Maryland School of Medicine/Baltimore (United States)
Xiang-Qun Guo, Xiamen Univ. (China) (United States)
Li Li, Univ. of Maryland School of Medicine/Baltimore (United States)
Henryk Szmacinski, Univ. of Maryland School of Medicine/Baltimore (United States)
Jeffrey Sipior, Univ. of Maryland School of Medicine/Baltimore (United States)
Joseph R. Lakowicz, Univ. of Maryland School of Medicine/Baltimore (United States)


Published in SPIE Proceedings Vol. 3256:
Advances in Optical Biophysics
Joseph R. Lakowicz; J. B. Alexander Ross, Editor(s)

© SPIE. Terms of Use
Back to Top