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Proceedings Paper

Probing the structure of macromolecules using microsecond time-resolved fluorescence of europium chelates
Author(s): Ewa Heyduk; Tomasz Heyduk
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Paper Abstract

Lanthanide chelates, due to their unique luminescence properties, offer a potential for improvement and simplification of fluorescence resonance energy transfer distance measurements. In this report we present a procedure for incorporation of highly-luminescent europium chelate into internal sites of ds DNA. Using this labeling strategy donor- acceptor labeled 20 bp DNA fragments were prepared containing a stretch of six A residues (A-tract) in the middle of the fragment. Distance measurement between the donor (europium chelate) and acceptor (Cy5) were performed and compared to measurements with a DNA fragment lacking the A-tract. Only small changes in measured distances were observed between these two DNA molecules. The implications of these results for existing models of A-tract induced DNA bending are discussed.

Paper Details

Date Published: 1 May 1998
PDF: 5 pages
Proc. SPIE 3256, Advances in Optical Biophysics, (1 May 1998); doi: 10.1117/12.307063
Show Author Affiliations
Ewa Heyduk, St. Louis Univ. School of Medicine (United States)
Tomasz Heyduk, St. Louis Univ. School of Medicine (United States)

Published in SPIE Proceedings Vol. 3256:
Advances in Optical Biophysics
Joseph R. Lakowicz; J. B. Alexander Ross, Editor(s)

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