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Proceedings Paper

Rapid identification of the multidrug resistance in the human leukemic cells by near-infrared Fourier transform Raman microspectroscopy
Author(s): Abdelilah Beljebbar; Hamid Morjani; Ganesh D. Sockalingum; Michel Manfait
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Paper Abstract

In this work, we have studied the cancer cell lines sharing a common feature: the multi-drug resistance where P- glycoprotein is responsible for the active efflux of the drug out of the cell. For this, we have used two types of cells, MDR-human leukemic K562 cells and non-MDR acute promyelocytic leukemic HL60 cells. The comparison between normalized micro FT-Raman spectra of resistant and sensitive K652 cells shows a decrease in the intensity of the amide I and III bands and a down shift of the amide I band. On the other hand, control experiments with HL60 cells do not show any remarkable changes. Analysis of micro-FT-Raman spectra by resolution enhancement methods and by chemometrics tools reveal further information concerning the conformational changes of the cell constituents involved in the expression of the MDR-phenotype.

Paper Details

Date Published: 24 April 1998
PDF: 4 pages
Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); doi: 10.1117/12.306106
Show Author Affiliations
Abdelilah Beljebbar, Univ. de Reims Champagne-Ardenne (Netherlands)
Hamid Morjani, Univ. de Reims Champagne-Ardenne (France)
Ganesh D. Sockalingum, Univ. de Reims Champagne-Ardenne (France)
Michel Manfait, Univ. de Reims Champagne-Ardenne (France)

Published in SPIE Proceedings Vol. 3257:
Infrared Spectroscopy: New Tool in Medicine
Henry H. Mantsch; Michael Jackson, Editor(s)

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