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Proceedings Paper

Raman spectroscopic study of leptospiral glycolipoprotein
Author(s): PeiDi Bao; Lang Bao; TianQuan Huang; XinMing Liu
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Paper Abstract

The Raman scattering spectra of two different samples of Leptospiral Glycoipoprotein (GLP-1 and GLP-2) which have different toxic effects have been obtained and investigated. Leptospirosis is one of the most harmful zoonosis. It is a serious public health issue in some area of Sichusan province. The two samples offer different structural informations of GLP molecules, it would be important to find the difference in contents, structures and the amino acid side - chains environment of the molecules between the two samples of GLP for understanding the different toxic effects. The intense Am I at 1651 cm-1 and weak Am III at 1283 cm-1 show that GLP-1 has a predominantly (alpha) -helix secondary structure. The intense Am I at 1674 cm-1 and intense Am III at 1246 cm-1 show that the conformation of GLP-2 has a high content of (Beta) - sheet and a low content of random - coil secondary structure. Strong Raman scattering occurs in the range 920- 980 cm-1, belong to the C-COO vibration and the stretching of the peptide backbone. The molecules of GLP-1 has trans-gauche-trans configuration of the C-S-S-C-C linkage and the molecules of GLP-2 has trans-gauche-gauche configuration of the C-C-S-S-C-C linkage. The intensity ratio of the two tyrosine liens at 830 cm-1 and 850 cm-1 is 1.1 and 1.23, indicate their tyrosine reduces environment respectively. Other side-chain environment in the two samples were discussed.

Paper Details

Date Published: 24 April 1998
PDF: 6 pages
Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); doi: 10.1117/12.306093
Show Author Affiliations
PeiDi Bao, Sichuan Univ. (China)
Lang Bao, West China Univ. of Medical Science (China)
TianQuan Huang, Sichuan Univ. (China)
XinMing Liu, Sichuan Univ. (China)

Published in SPIE Proceedings Vol. 3257:
Infrared Spectroscopy: New Tool in Medicine
Henry H. Mantsch; Michael Jackson, Editor(s)

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