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Proceedings Paper

Imaging of human colon carcinoma thin sections by FT-IR microspectrometry
Author(s): Peter Lasch; Wolfgang Waesche; W. J. McCarthy; Gerhard J. Mueller; Dieter Naumann
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Paper Abstract

FTIR microspectroscopic maps of unstained colon carcinoma thin sections were obtained on a conventional IR microscope equipped with an automatic x, y stage, or alternatively by using a MCT focal plane array detector system. IR data were analyzed by different image re-assembling techniques. One main goal of the present study was to test the influence of different spectra data compression approaches on the quality of the FTIR images. The images, re-assembled by Principal component analysis (PCA) on the basis of spectral information available from the fingerprint region exhibited an excellent image contrast confirming standard histo- pathological examinations. The second approach included a systematic search for spectral windows which were supposed to contain the relevant information, necessary for spectra classification and identification. Data from these spectral windows were analyzed by an ANN and output data were utilized for image construction. In contrast to the PCA approach, the image contrast was lower although the main morphological structures were exactly classified. From the spectroscopic point of view, the spectral feature selection method delivered useful information which could be discussed in terms of structural alternations upon carcinogenesis.

Paper Details

Date Published: 24 April 1998
PDF: 12 pages
Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); doi: 10.1117/12.306083
Show Author Affiliations
Peter Lasch, Robert Kock-Institut (Germany)
Wolfgang Waesche, Laser- und Medizin- Technologie GmbH (Germany)
W. J. McCarthy, Bio-Rad Labs. (United States)
Gerhard J. Mueller, Laser- und Medizin- Technologie GmbH (Germany)
Dieter Naumann, Robert Koch-Institut (Germany)

Published in SPIE Proceedings Vol. 3257:
Infrared Spectroscopy: New Tool in Medicine
Henry H. Mantsch; Michael Jackson, Editor(s)

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