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Proceedings Paper

Infrared spectroscopic imaging as a tool for pathology
Author(s): Linda H. Kidder; Abigail S. Haka; Patrick J. Faustino; David S. Lester; Ira W. Levin; E. Neil Lewis
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Paper Abstract

Conventional Fourier transform IR spectroscopy has proven to be an invaluable research and diagnostic approach for the study of a wide range of biomedical problems. In this article we describe a new biomedical imaging method which integrates high-resolution IR spectroscopy with high- definition digital imaging. The continuing development and commercialization of long-wavelength IR cameras or focal- plane arrays has been a key enabling technology. These imaging systems are capable of rapidly generating chemically specific images from a variety of unstained biological tissue and cells. Image contrast is intrinsic to the sample and is determined only by its biochemical composition. In addition, data from a single experiment can be digitally manipulated to produce numerous images of the same sample, for which different spatial and biochemical properties are emphasized. We present data demonstrating the potential of the technique to generate spectroscopic signatures and images from single human breast cells.

Paper Details

Date Published: 24 April 1998
PDF: 9 pages
Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); doi: 10.1117/12.306081
Show Author Affiliations
Linda H. Kidder, National Institutes of Health (United States)
Abigail S. Haka, National Institutes of Health (United States)
Patrick J. Faustino, Food and Drug Administration (United States)
David S. Lester, Food and Drug Administration (United States)
Ira W. Levin, National Institutes of Health (United States)
E. Neil Lewis, National Institutes of Health (United States)

Published in SPIE Proceedings Vol. 3257:
Infrared Spectroscopy: New Tool in Medicine
Henry H. Mantsch; Michael Jackson, Editor(s)

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