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Proceedings Paper

Multicomponent assay for blood substrates in human sera and haemolysed blood by mid-infrared spectroscopy
Author(s): Gerhard H. Werner; Dirk Boecker; Hans-Peter Haar; Hans-Juergen Kuhr; Reinhold Mischler
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Paper Abstract

A method of multicomponent analysis for blood substrates based on FTIR spectroscopy is described. Venous and capillary blood was used as a matrix, with sample volumes as small as 1 (mu) l. The spectra were obtained by transmission and diffuse reflection measurements from dried samples on a disposable carrier. The mean square prediction errors for 90 blood samples form different patients, calculated by cross- validation, were found to be 18 mg/dl for glucose, 17.5 mg/dl for cholesterol, 23.5 mg/dl for triglycerides, and 0.77 g/dl for haemoglobin. The mean square prediction errors for serum analyses are smaller: for a population of more than 200 serum samples we found 8 mg/dl for cholesterol, 14.9 mg/dl for triglycerides, 0.23 g/dl for total proteins, and 8.7 mg/dl for urea. The in-series standard error for the glucose concentration was low as 2.2 mg/dl. The combined spectroscopic and biological error for glucose was found to be 7 mg/dl. This value includes the 'matrix effect'. In independent serum samples glucose concentrations were measured with a total error of 16 mg/dl.

Paper Details

Date Published: 24 April 1998
PDF: 10 pages
Proc. SPIE 3257, Infrared Spectroscopy: New Tool in Medicine, (24 April 1998); doi: 10.1117/12.306074
Show Author Affiliations
Gerhard H. Werner, Boehringer Mannheim GmbH (Germany)
Dirk Boecker, Boehringer Mannheim GmbH (United States)
Hans-Peter Haar, Boehringer Mannheim GmbH (Germany)
Hans-Juergen Kuhr, Boehringer Mannheim GmbH (Germany)
Reinhold Mischler, Boehringer Mannheim GmbH (Germany)

Published in SPIE Proceedings Vol. 3257:
Infrared Spectroscopy: New Tool in Medicine
Henry H. Mantsch; Michael Jackson, Editor(s)

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