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Proceedings Paper

Two-dimensional fluorescence lifetime imaging for in-vitro and in-vivo application
Author(s): Paul M. W. French; Mark J. Dayel; Keith Dowling; Sam C. W. Hyde; M. John Lever; P. Vourdas; Anthony K. L. Dymoke-Bradshaw; Jonathan D. Hares
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Paper Abstract

We report the development of a fluorescence lifetime imaging (FLIM) system based on a time-gated image intensifier and solid-state laser amplifier with a system response of < 100 ps. We have sued this system to image lifetimes as short as 100 ps and to image changes in the environment of a fluorescent phantom, causing lifetime differences less than 10 ps. The versatility of this FLIM system has been demonstrated by measuring both the temporal and spectral profiles of multiple fluorescent samples in a single acquisition. Fluorescence lifetime imaging of tissue constituents has also been carried out and first results suggest that this technique can provide a means of distinguishing between different tissue constituents.

Paper Details

Date Published: 16 April 1998
PDF: 8 pages
Proc. SPIE 3250, Optical Biopsy II, (16 April 1998); doi: 10.1117/12.305374
Show Author Affiliations
Paul M. W. French, Imperial College of Science, Technology and Medicine (United Kingdom)
Mark J. Dayel, Imperial College of Science, Technology and Medicine (United Kingdom)
Keith Dowling, Imperial College of Science, Technology and Medicine (United Kingdom)
Sam C. W. Hyde, Imperial College of Science, Technology and Medicine (United Kingdom)
M. John Lever, Imperial College of Science, Technology and Medicine (United Kingdom)
P. Vourdas, Imperial College of Science, Technology and Medicine (United Kingdom)
Anthony K. L. Dymoke-Bradshaw, Kentech Instruments Ltd. (United Kingdom)
Jonathan D. Hares, Kentech Instruments Ltd. (United Kingdom)


Published in SPIE Proceedings Vol. 3250:
Optical Biopsy II
Robert R. Alfano, Editor(s)

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