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Proceedings Paper

Miniaturization of multifunctional biosensors with enzyme-immobilized beads
Author(s): Yuji Murakami; Kenji Yokoyama; Eiichi Tamiya
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Paper Abstract

We propose a novel method for individual immobilization. Biomaterials were first immobilized enlarges the size of biomaterials to the size of sensor element. Then the support is arranged on the sensor element by self-assembling. The element was microfabricated to have a microstructure for self-assembling. When both size of biomaterial and the element are the same, self-assemble is expected to give one- to-one correspondence, and then individual response. Mixed glass beads immobilized each of various enzyme were put near the pits fabricated one chip and the chip was slanted to roll the beads into the pits. When the beads immobilized only with peroxidase were arranged, the addition of luminol and hydrogen peroxide gave chemiluminescence at almost every site. Next, the beads immobilized only with glucose oxidase as dummy enzyme were mixed with HRP-beads and arranged to the sites. Addition of substrate gave limited number of luminescence-giving sites, though every site had an enzyme- immobilized bead. These results show that two kinds of enzymes were separately arranged in the site in one-to-one correspondence.

Paper Details

Date Published: 26 March 1998
PDF: 4 pages
Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); doi: 10.1117/12.304381
Show Author Affiliations
Yuji Murakami, Japan Advanced Institute of Science and Technology (Japan)
Kenji Yokoyama, Japan Advanced Institute of Science and Technology (Japan)
Eiichi Tamiya, Japan Advanced Institute of Science and Technology (Japan)


Published in SPIE Proceedings Vol. 3258:
Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications
Paul Lee Gourley, Editor(s)

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