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Proceedings Paper

Ultrasensitive detection of red blood cell lysing in a microfabricated semiconductor laser cavity
Author(s): Paul Lee Gourley; T. French; Anthony E. McDonald; E. A. Shields; Mark F. Gourley
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Paper Abstract

In this paper we report investigations of semiconductor laser microcavities for use in detecting changes of human blood cells during lysing. By studying the spectral before and during mixing of blood fluids with di-ionized water, we are able to qualify the cell shape and concentration of hemoglobin in real time during the dynamical process of lysing. We find that the spectra can detect subtle changes that are orders of magnitude smaller than can be observed by standard optical microscopy. Such sensitivity in observing cell structural changes has implications for measuring cell fragility, monitoring apoptitic events in real time, development of photosensitizers for photodynamic therapy, and in-vitro micromanipulation techniques.

Paper Details

Date Published: 26 March 1998
PDF: 12 pages
Proc. SPIE 3258, Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications, (26 March 1998); doi: 10.1117/12.304380
Show Author Affiliations
Paul Lee Gourley, Sandia National Labs. (United States)
T. French, Sandia National Labs. (United States)
Anthony E. McDonald, Sandia National Labs. (United States)
E. A. Shields, Sandia National Labs. (United States)
Mark F. Gourley, Washington Hospital Ctr. (United States)

Published in SPIE Proceedings Vol. 3258:
Micro- and Nanofabricated Structures and Devices for Biomedical Environmental Applications
Paul Lee Gourley, Editor(s)

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