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Proceedings Paper

Manipulation of micro-objects by means of a focused laser beam
Author(s): Alexandr Jonas; Libor Sramek; Miroslav Liska; Pavel Zemanek
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Paper Abstract

Force exerted on a micro-object by the focused laser beam can be as high as the gravity force acting on the object or even significantly higher. This implies that the laser light can be used to manipulate small objects (range size approximately 0.1 - 100 micrometer) within suitable immersion medium. This possibility is of a great practical importance, for example, for microbiology and molecular biology (manipulation with single living cells, cell organelles, chromosomes, etc.) as well as for micromachinery and other technical branches. We use a ray-optics-based model to determine the magnitude of forces exerted by laser light as the functions of laser beam, object and surrounding medium parameters. We study the influence of these parameters on total force in order to find the optimal parameter combination for the most effective manipulation. We have employed these theoretical results in practice and succeeded in building up a 3-D laser trap which we use to manipulate divinylbenzen spherical particles (10 - 35 micrometer sized) and also irregularly shaped living protozoa cells in water medium.

Paper Details

Date Published: 1 January 1998
PDF: 7 pages
Proc. SPIE 3320, Tenth Polish-Czech-Slovak Optical Conference: Wave and Quantum Aspects of Contemporary Optics, (1 January 1998); doi: 10.1117/12.301324
Show Author Affiliations
Alexandr Jonas, Technical Univ. of Brno (Czech Republic)
Libor Sramek, Technical Univ. of Brno (Czech Republic)
Miroslav Liska, Technical Univ. of Brno (Czech Republic)
Pavel Zemanek, Institute of Scientific Instruments (Czech Republic)

Published in SPIE Proceedings Vol. 3320:
Tenth Polish-Czech-Slovak Optical Conference: Wave and Quantum Aspects of Contemporary Optics
Jerzy Nowak; Marek Zajac, Editor(s)

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