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Proceedings Paper

NIR spectroscopic investigation of m. vastus lateralis in patients with mitochondrial myopathies as detected by respirometric investigation of mitochondrial function in skinned fibers
Author(s): Frank N. Gellerich; Tobias Mueller; Shoko Nioka; Katrin Hertel; Wilhelm J. Schulte-Mattler; Stephan Zierz; Britton Chance
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Paper Abstract

Noninvasive measurement of changes in oxygenation of human skeletal muscle can be done with a dual-wavelength near infrared (NIR) spectrophotometer. This allows a noninvasive investigation of muscle mitochondria. An exercise protocol was developed to study the load dependent changes in oxygenation of m. vastus lateralis of myopathic patients. On a bicycle ergometer exercise was done periodically. One period consisted of 1.5 min exercise followed by 3 min rest. Work load in the first period was 20 W, and was increased by 10 W for each subsequent period until maximal work load was reached. In 12 healthy volunteers we observed oxygenation of muscle during periods of low work load (warm-up effect). During periods of high work load the muscle deoxygenated. The work load at transition from oxygenation to deoxygenation (deoxygenation threshold) in controls was 75 W. In 3 patients with myopathies, in addition to NIR- spectroscopy, function of mitochondria of specimen of m. vastus lateralis was investigated biochemically. Muscle fibers were skinned with saponin and investigated with high resolution respirometry and multiple substrate-inhibitor- titration. Mitochondrial function was impaired in patients who had abnormal findings in NIR spectroscopy.

Paper Details

Date Published: 1 January 1998
PDF: 12 pages
Proc. SPIE 3194, Photon Propagation in Tissues III, (1 January 1998); doi: 10.1117/12.301043
Show Author Affiliations
Frank N. Gellerich, Martin-Luther-Univ. Halle-Wittenberg (Germany)
Tobias Mueller, Martin-Luther-Univ. Halle-Wittenberg (Germany)
Shoko Nioka, Univ. of Pennsylvania (United States)
Katrin Hertel, Martin-Luther-Univ. Halle-Wittenberg (Germany)
Wilhelm J. Schulte-Mattler, Martin-Luther-Univ. Halle-Wittenberg (Germany)
Stephan Zierz, Martin-Luther-Univ. Halle-Wittenberg (Germany)
Britton Chance, Univ. of Pennsylvania (United States)

Published in SPIE Proceedings Vol. 3194:
Photon Propagation in Tissues III
David A. Benaron; Britton Chance; Marco Ferrari, Editor(s)

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