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Proceedings Paper

X-ray microscopy with compact pulsed sources
Author(s): Martin C. Richardson; Masataka Kado; David Scott Torres; Yoshimasa Yamamoto; Herman Friedman; Jayshree Rajyaguru; Michael J. Muszynski
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Paper Abstract

X-ray microscopy inherently possesses characteristics complementary to optical and electron microscopy. Short wavelength x-ray radiation, especially in the so-called 'water window', permits a twenty-fold improvement in spatial resolution over optical microscopy while preserving a depth of field large enough to image whole biological specimens int heir natural state. Whereas electron microscopy can access atomic-scale resolution,this can only be applied to biological and medical specimens at the expense of detrimental preparation procedures that preclude real-time analysis of structural changes in living organisms. We describe progress being made in an x-ray imaging technology that provides high-resolution single frame x-ray images of in-vitro specimens captured in a time sufficiently short that any radiation damage mechanisms to the structure are not recorded. Several different biology and medical research groups find this type of microscopy particularly well-suited to the detailed analysis of sub-cellular features, and to the study of live organisms subjected to various forms of external stimuli. This technology utilizes bright x-ray sources produced by compact pulse laser systems. The incorporation of advanced x-ray optical and electron-optical systems will lead to the development of a compact, real-time x-ray microscope, having a broad range of applications.

Paper Details

Date Published: 1 March 1998
PDF: 7 pages
Proc. SPIE 3240, 26th AIPR Workshop: Exploiting New Image Sources and Sensors, (1 March 1998); doi: 10.1117/12.300073
Show Author Affiliations
Martin C. Richardson, CREOL/Univ. of Central Florida (United States)
Masataka Kado, CREOL/Univ. of Central Florida (United States)
David Scott Torres, CREOL/Univ. of Central Florida (United States)
Yoshimasa Yamamoto, Univ. of South Florida (United States)
Herman Friedman, Univ. of South Florida (United States)
Jayshree Rajyaguru, Arnold Palmer Hospital for Children and Women (United States)
Michael J. Muszynski, Arnold Palmer Hospital for Children and Women (United States)


Published in SPIE Proceedings Vol. 3240:
26th AIPR Workshop: Exploiting New Image Sources and Sensors
J. Michael Selander, Editor(s)

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