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Proceedings Paper

Effects of bromodeoxyuridine on DNA and cytoskeleton of primitive blood cells differentiating after exposure in a chick embryo in vivo
Author(s): Bozena Novotna; Irena Linhartova; Vladimir Viklicky
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Paper Abstract

Three-day-old chick embryos were exposed intra-amniotically to bromodeoxyuridine within the range of teratogenic doses. Using comet assay, a significant damage of DNA was demonstrated in blood cells 3 h after the treatment. While the damage seemed to be partially repaired within 12 h, new peak of DNA fragmentation detected on incubation day 4 implied an apoptotic elimination of impaired cells. More frequent occurrence of macrophages in blood samples from BrdU treated embryos supports this assumption. The differentiating blood cells, however, did not exhibit any remarkable injury of cytoskeleton biogenesis. Nevertheless, an improved experimental procedure revealed the existence of intermediate 'wreath' stage preceding the consolidation of tubulin bundles into marginal band of chicken erythroblasts already within the course of embryonic period. The more, even the mature cells of primitive erhthroid series retained the visible bundles of radial microtubules attached to MTOC. Actin labeling disclosed in many primitive erythroblasts the special lace arrangement of microfilaments growing from nucleus surface while the rest of cells exhibited only a diffuse staining through cytoplasm, concentrated sometimes in area of marginal band. Such distribution was characteristic for mature form of primitive and definitive erythrocytes. The expression of vimentin in erythroid cells was very weak and quite different from patterns of adult definitive erythrocytes. The labeling was noticed only around the nucleus till incubation day 10 when implication of fiber growth through cytoplasm was detected. Conventional hematological analysis performed on incubation day 10 revealed in blood of BrdU treated embryos the lower incidence of definitive erythrocytes in favor of immature forms resulting probably from death of cells in consequence of primary DNA damage. Such effect could be associated with development of myelodysplastic syndrome in later life.

Paper Details

Date Published: 29 December 1997
PDF: 12 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297983
Show Author Affiliations
Bozena Novotna, Institute of Experimental Medicine (Czech Republic)
Irena Linhartova, Institute of Molecular Genetics (Czech Republic)
Vladimir Viklicky, Institute of Molecular Genetics (Czech Republic)

Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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