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Proceedings Paper

Mapping the distribution of zinc phthalocyanine derivatives in EAhy 926 cells using Raman microscopy
Author(s): Tami L. Freeman; Sarah E. Cope; Mark R. Stringer; Janet E. Cruse-Sawyer; Stanley B. Brown; David N. Batchelder
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Paper Abstract

We have applied Raman spectroscopy to the study of the distribution within cells of photosensitizing compounds designed for use in photodynamic therapy. A human endothelial hybridoma cell-line was incubated in the presence of substituted zinc phthalocyanines, with the cells subsequently fixed in formalin. Microscopic Raman analysis was performed using 782 nm excitation in order to avoid photosensitizer absorption, thereby minimizing both the background fluorescence signal and the generation of cytotoxic species. A method of Raman mapping is described and we demonstrate that it is possible to identify the distribution of photosensitizer within the cell. The results indicate that sub-cellular photosensitizer localization is dependent upon both molecular structure and incubation time.

Paper Details

Date Published: 29 December 1997
PDF: 9 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297981
Show Author Affiliations
Tami L. Freeman, Research School of Medicine/Univ. of Leeds (United Kingdom)
Sarah E. Cope, Research School of Medicine/Univ. of Leeds (United Kingdom)
Mark R. Stringer, Research School of Medicine/Univ. of Leeds (United Kingdom)
Janet E. Cruse-Sawyer, Research School of Medicine/Univ. of Leeds (United Kingdom)
Stanley B. Brown, Univ. of Leeds (United Kingdom)
David N. Batchelder, Univ. of Leeds (United Kingdom)


Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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