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Proceedings Paper

Evaluation of interacellular tamoxifen-induced fluorescence in tamoxifen-resistant human breast adenocarcinoma cells
Author(s): Nathalie Bachmann; Muriel Barberi-Heyob; Marie-Pierre Gramain; Corinne Bour; Sophie Marchal; Robert Michel Parache; Francois H. Guillemin; Jean-Louis Merlin
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Paper Abstract

A tamoxifen resistant cell line (MCF7TAM) was established from tamoxifen sensitive MCF-7 human adenocarcinoma cells expressing estrogen receptors. The resistant cell line was found to express estrogen receptors to similar level as the parent cell line but the receptors were found to be altered, having lost their ability to bind estradiol or tamoxifen. The fluorescence of eosin-tamoxifen ionic association was used to investigate intracellular location of tamoxifen in both sensitive and resistant cell lines. Fluorescence emission spectra of eosin, tamoxifen and eosin-tamoxifen complex ((lambda) exc equals 480 nm) were analyzed and showed that maximal fluorescence intensity of the complex ((lambda) em equals 540 nm) was four times higher than that of eosin alone while tamoxifen alone did not emit any fluorescence in this spectral range. In MCF-7 cells, tamoxifen was found to be diffusively located in the cytoplasm and nuclear fluorescence intensity was significantly lower. No difference was observed in fluorescence intensity or location in tamoxifen resistant cells, although it has been previously correlated with clinical responsiveness. Improvement of this fluorescence microscopy methodology appears necessary to provide accurate results taking into account the complexity of tamoxifen resistance molecular pathways.

Paper Details

Date Published: 29 December 1997
PDF: 5 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297980
Show Author Affiliations
Nathalie Bachmann, Ctr. Alexis Vautrin (France)
Muriel Barberi-Heyob, Ctr. Alexis Vautrin (France)
Marie-Pierre Gramain, Ctr. Alexis Vautrin (France)
Corinne Bour, Ctr. Alexis Vautrin (France)
Sophie Marchal, Ctr. Alexis Vautrin (France)
Robert Michel Parache, Ctr. Alexis Vautrin (France)
Francois H. Guillemin, Ctr. Alexis Vautrin (France)
Jean-Louis Merlin, Ctr. Alexis Vautrin (France)


Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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