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Proceedings Paper

Near-field optical microscopy for subwavelength investigation of biological structures
Author(s): Fabienne D. Marquis-Weible; Claude Philipona; Patrick Lambelet; Abdeljlail Sayah; Michael Pfeffer
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Paper Abstract

Applications of scanning near field optical microscopy to the imaging and the structuring of biomolecules is presented. The paper introduces the technique and presents images of a test grating, showing the potential of the technique for subwavelength imaging in air as well as in water. A sub- micrometer size fiber tip characterized by its high transmission is presented as an interesting probe for near field fluorescence microscopy. The potential of the technique is exploited to detect bioactive molecules that have been marked with a fluorescent probe and photoimmobilized onto a glass substrate using near field UV-activation of a photolinker.

Paper Details

Date Published: 29 December 1997
PDF: 9 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297979
Show Author Affiliations
Fabienne D. Marquis-Weible, Ecole Polytechnique Federale de Lausanne (Switzerland)
Claude Philipona, Ecole Polytechnique Federale de Lausanne (Switzerland)
Patrick Lambelet, Ecole Polytechnique Federale de Lausanne (Switzerland)
Abdeljlail Sayah, Ecole Polytechnique Federale de Lausanne (Switzerland)
Michael Pfeffer, Ecole Polytechnique Federale de Lausanne (Switzerland)


Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg M.D.; Pierre M. Viallet, Editor(s)

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