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Proceedings Paper

Near-field optical microscopy of bacteria thin sections
Author(s): Nikolai P. Konnov; Vil B. Baiburin; Anatolyi A. Shcherbakov; Alina N. Malakhaeva; Yuri P. Volkov
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Paper Abstract

Whole bacteria as well as thin sections were investigated in our laboratory by means of near field scanning optical microscope (NSOM). The main problem in NSOM operation is a control of distance between microscopy tip and sample surface. The bacteria thin section is a more preferable sample for NSOM investigation because of its flat surface. For increasing of thin sections' image contrast were used different light microscopy stainers (Eosin, Hematoxylin, etc.). We obtained images of thin sections of plague (Y. Pestis EV) and cholera (V. Cholerae). Lateral resolution in the investigation is about 300 angstroms.

Paper Details

Date Published: 29 December 1997
PDF: 3 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297978
Show Author Affiliations
Nikolai P. Konnov, Russian Research Anti-Plague Institute Microbe (Russia)
Vil B. Baiburin, Saratov Technical Univ. (Russia)
Anatolyi A. Shcherbakov, Russian Research Anti-Plague Institute Microbe (Russia)
Alina N. Malakhaeva, Russian Research Anti-Plague Institute Microbe (Russia)
Yuri P. Volkov, Saratov Technical Univ. (Russia)


Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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