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Proceedings Paper

Intracellular dynamics with the phase microscope Airyscan
Author(s): Vladimir P. Tychinsky; Elena V. Perevedentseva; Tatiana V. Vyshenskaia; Georgy E. Kufal
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Paper Abstract

Investigation of intracellular dynamics of Allium cepa inner epidermal cells are described. The applicability of the method for quantitative estimation of spatio-temporal phase fluctuations and the effect due to external factors is discussed. The analysis of time-sampled series allows one to detect the regions of various motility in cytoplasm. The intense Fourier-spectra harmonics in 0.2 - 8 Hz interval were observed inside a cell wall and cytoplasm. Regularly spaced 2- to 4-s long batches of 100-ms pulses at cell-wall sites are recorded. The phase-fluctuation intensity decreased and the frequencies of certain harmonics were shifted with lowering temperature. The advantages and specific features of the method are discussed.

Paper Details

Date Published: 29 December 1997
PDF: 6 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297975
Show Author Affiliations
Vladimir P. Tychinsky, Moscow State Institute for Radio Engineering, Electronics, and Automation (Russia)
Elena V. Perevedentseva, Moscow State Institute for Radio Engineering, Electronics, and Automation (Russia)
Tatiana V. Vyshenskaia, Moscow State Institute for Radio Engineering, Electronics, and Automation (Russia)
Georgy E. Kufal, Moscow State Institute for Radio Engineering, Electronics, and Automation (Russia)


Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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