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Proceedings Paper

FTIR microspectroscopy of tissues for in-vivo and in-vitro cancer diagnostics
Author(s): Wolfgang Waesche; Uwe Bindig; Gerhard J. Mueller; P. Frege; Ulrich M. Gross
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Paper Abstract

FT-IR-microspectroscopic mapping technique has been used in combination with imaging methods for characterizing thin tissue sections of human adenocarcinomas of the colon and rectum as well as carcinomas of the breast. This paper presents results of microspectroscopic measurements in vitro of 10 micrometer cryosections of healthy and tumor tissue samples of gastro-intestinal and gastro-oesophageal origin by using a minimal spatial resolution of 100 micrometers squared. This technique is not only able to detect the amount of collagen, lipids and tissue related features as well as different substructures of the tissue samples, it could also be used for the differentiation between healthy and tumor tissue. The IR-maps based on the ratio of intensities of selected wavenumbers were compared with parallel cut and HE stained cryosections which were judged by a pathologist. The method is based on differences in IR-spectra of tissues which have been already described in the literature. Several papers have shown that the main differences are to be expected in the so-called 'fingerprint region' (1500 - 1000 cm-1). Additional spectroscopic changes arise from carbonyl/amide vibrational modes. Measurements were carried out using transmission, attenuated total reflection and spatial reflectance infrared spectroscopy. IR-maps of healthy and tumor tissue specimen are presented and discussed. Different modes of spectra acquisition (transmission, ATR, diffuse reflectance) are compared. The aim of the investigations is the determination of suitable wavelengths to distinguish between healthy and tumor epithelia tissue for tumor diagnostic with an endoscopic approach in vivo.

Paper Details

Date Published: 29 December 1997
PDF: 7 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297955
Show Author Affiliations
Wolfgang Waesche, Laser- und Medizin-Technologie gGmbH (Germany)
Uwe Bindig, Universitatsklinikum Benjamin Franklin der Freien Univ. Berlin (Germany)
Gerhard J. Mueller, Laser- un Medizin Technologie gGmbH (Germany) and Universitatsklinikum Benjamin Franklin der Freien (Germany)
P. Frege, Universitatsklinikum Benjamin Franklin der Freien Univ. Berlin (Germany)
Ulrich M. Gross, Universitatsklinikum Benjamin Franklin der Freie Univ. Berlin (Germany)

Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg M.D.; Pierre M. Viallet, Editor(s)

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