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Proceedings Paper

Use of the polarization vector in modeling tissue fluorescence: theoretical and experimental comparison
Author(s): Ourania Panou-Diamanti; Nikolaos K. Uzunoglou; A. Vasiliou; Giannis Zacharakis; George Filippidis; Theodore G. Papazoglou; Dimitrios Koutsouris
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Paper Abstract

This paper describes an innovative approach to tissue fluorescence as a phenomenon of inelastic scattering of electromagnetic radiation. A general formulation of the polarization vector of the medium is used in order to describe the fluorescence inelastic scattering process. The general method described, is applied to two different tissue models. In the first model, tissue is represented as a single infinitely thick, homogeneous layer, under plane wave excitation. The second model used is based on a picture of tissue as a single dielectric layer, under pulse excitation. In both cases, fluorophores and absorbing species are assumed to be homogeneously distributed inside tissue. The mathematical techniques used together with the fully evaluated theoretical results are presented. Additionally, experimental measurements were performed on collagen gels which contained various dyes in order to evaluate and validate our mathematical modeling.

Paper Details

Date Published: 29 December 1997
PDF: 11 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297953
Show Author Affiliations
Ourania Panou-Diamanti, National Technical Univ. of Athens (Greece)
Nikolaos K. Uzunoglou, National Technical Univ. of Athens (Greece)
A. Vasiliou, Institute of Electronic Structure and Laser/Foundation on Research and Technology-Hellas (Greece)
Giannis Zacharakis, Institute of Electronic Structure and Laser/Foundation on Research and Technology-Hellas (Greece)
George Filippidis, Institute of Electronic Structure and Laser/Foundation on Research and Technology-Hellas (Greece)
Theodore G. Papazoglou, Institute of Electronic Structure and Laser/Foundation on Research and Technology-Hellas (Greece)
Dimitrios Koutsouris, National Technical Univ. of Athens (Greece)


Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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