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Proceedings Paper

Colon cancer diagnosis using fluorescence spectroscopy and fluorescence imaging technique
Author(s): Dido M. Yova; Vassilis Atlamazoglou; P. Davaris; Nikolaos Kavantzas; Spyros Loukas
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Paper Abstract

It is well known that fluorescence spectroscopy can provide information about the differences in the concentration of chromophores in healthy and cancerous tissues. The tumor detection potential can be enhanced by using exogenous fluorescent agents with selective accumulation in cancerous tissue. In this study healthy and cancerous human colon tissue samples were obtained after colon surgery. Excitation-emission matrices were collected using a fluorescence spectrometer. The optimum excitation wavelength lied at 340 nm. After the acquisition of autofluorescence spectra, the samples were incubated in a solution of 4 (mu) g/ml of Rhodamine analogs. Rhodamine B, Rhodamine 6G and three recently synthesized analogs, were used. For the acquisition of fluorescence images, an endoscopic imaging system was developed. Fluorescence imaging with the concomitant use of Rhodamine analogs revealed a remarkable differentiation of cancerous from healthy colonic mucosa.

Paper Details

Date Published: 29 December 1997
PDF: 12 pages
Proc. SPIE 3197, Optical Biopsies and Microscopic Techniques II, (29 December 1997); doi: 10.1117/12.297946
Show Author Affiliations
Dido M. Yova, National Technical Univ. of Athens (Greece)
Vassilis Atlamazoglou, National Technical Univ. of Athens (Greece)
P. Davaris, Univ. of Athens Medical School (Greece)
Nikolaos Kavantzas, Univ. of Athens Medical School (Greece)
Spyros Loukas, National Ctr. for Scientific Research Demokritos (Greece)

Published in SPIE Proceedings Vol. 3197:
Optical Biopsies and Microscopic Techniques II
Irving J. Bigio; Herbert Schneckenburger; Jan Slavik; Katarina Svanberg; Pierre M. Viallet, Editor(s)

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