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Proceedings Paper

Antigen recognition by T-lymphocyte studied with an optical trap
Author(s): Xunbin Wei; Tatiana B. Krasieva; Paul A. Negulescu; Zhanxiang Zhang; Chung-Ho Sun; Michael W. Berns; Gregory J. Sonek; Michael D. Cahalan; Bruce J. Tromberg
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Paper Abstract

T-cell contact with antigen-presenting B cells initiates an activation cascade which includes an increase in T-cell intracellular calcium and leads to T-cell proliferation and differentiation. We studied cell-cell contact requirements for T-cell activation using an optical trap to control the orientation of T-cell/B-cell pairs and fluorescence microscopy to measure subsequent T-cell(Ca2+)i response. B cells or beads coated with antibodies to the T- cell receptor are trapped with a titanium-sapphire laser and placed at different locations along the T-cell, which has a polarized appearance defined by the shape and direction of crawling. T-cell (Ca2+)i is detected as an emission shift from the combination of fura-red and oregon- green, two cytoplasmic (Ca2+)i indicators. T- cells which are presented antigen at the leading edge have a higher probability of responding and a shorter latency of response than those contacting B-cells or beads with their trailing end.

Paper Details

Date Published: 13 January 1998
PDF: 6 pages
Proc. SPIE 3196, Optical and Imaging Techniques for Biomonitoring III, (13 January 1998); doi: 10.1117/12.297930
Show Author Affiliations
Xunbin Wei, Univ. of California/Irvine and Beckman Laser Institute (United States)
Tatiana B. Krasieva, Beckman Laser Institute and Medical Clinic (United States)
Paul A. Negulescu, Univ. of California/Irvine (United States)
Zhanxiang Zhang, Beckman Laser Institute and Medical Clinic and Univ. of California/Irvine (United States)
Chung-Ho Sun, Beckman Laser Institute and Medical Clinic (United States)
Michael W. Berns, Beckman Laser Institute and Medical Clinic (United States)
Gregory J. Sonek, Beckman Laser Institute and Medical Clinic and Univ. of California/Irvine (United States)
Michael D. Cahalan, Univ. of California/Irvine (United States)
Bruce J. Tromberg, Univ. of California/Irvine and Beckman Laser Institute and Medical Clinic (United States)


Published in SPIE Proceedings Vol. 3196:
Optical and Imaging Techniques for Biomonitoring III
Hans-Jochen Foth; Renato Marchesini; Halina Podbielska; Abraham Katzir, Editor(s)

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