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Proceedings Paper

High-resolution whole field fluorescence lifetime imaging of fluorophore distribution and environment
Author(s): Mark J. Dayel; Keith Dowling; Sam C. W. Hyde; Christopher Dainty; Paul M. W. French; P. Vourdas; M. John Lever; Anthony K. L. Dymoke-Bradshaw; Jonathan D. Hares; Paul A. Kellett
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Paper Abstract

We report the development of a high temporal resolution fluorescence lifetime imaging (FLIM) system suing a time- gated image intensifier to provide whole field FLIM images. The gate width has been optimized to 110 ps, and changes in the environment of a fluorescent phantom, causing lifetime differences of 20 ps, have been detected. Environmental changes of the fluorescent indicator, Lucifer Yellow, have been sensed by measuring changes in its fluorescence lifetime in the presence of the protein albumin. We also present provisional fluorescence lifetime images of tissue constituents.

Paper Details

Date Published: 13 January 1998
PDF: 7 pages
Proc. SPIE 3196, Optical and Imaging Techniques for Biomonitoring III, (13 January 1998); doi: 10.1117/12.297925
Show Author Affiliations
Mark J. Dayel, Imperial College of Science, Technology and Medicine (United Kingdom)
Keith Dowling, Imperial College of Science, Technology and Medicine (United Kingdom)
Sam C. W. Hyde, Imperial College of Science, Technology and Medicine (United Kingdom)
Christopher Dainty, Imperial College of Science, Technology and Medicine (United Kingdom)
Paul M. W. French, Imperial College of Science, Technology and Medicine (United Kingdom)
P. Vourdas, Imperial College of Science, Technology and Medicine (United Kingdom)
M. John Lever, Imperial College of Science, Technology and Medicine (United Kingdom)
Anthony K. L. Dymoke-Bradshaw, Kentech Instruments Ltd. (United Kingdom)
Jonathan D. Hares, Kentech Instruments Ltd. (United Kingdom)
Paul A. Kellett, Kentech Instruments Ltd. (United Kingdom)


Published in SPIE Proceedings Vol. 3196:
Optical and Imaging Techniques for Biomonitoring III
Hans-Jochen Foth; Renato Marchesini; Halina Podbielska; Abraham Katzir, Editor(s)

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