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Proceedings Paper

Porphyrin binding with blood cells
Author(s): Vladimir P. Zorin; Ivan I. Khludeyev; Valery P. Savitsky; Sergey B. Mel'nov; Nina D. Kochubeyeva
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Paper Abstract

Using fluorescence activated cell sorting we have compared the binding of a number of porphyrins with different polarity by blood cells. According to pigment level blood cells may be arranged in order granulocytes greater than or equal to monocytes greater than lymphocytes greater than erythrocytes. Cellular accumulation of selected porphyrins in blood cells was remarkably different. The equilibrium level of chlorin e6 dimethylester in blood cells was about 15 times higher compared with chlorin e6. As a result, the percentage of pigment binding by blood cells varied from 0% (of total amount) in the case of polar pigments to about 50% for moderately apolar porphyrins. The results obtained show that pigment binding to blood cells may be of certain value when the pharmacokinetic behavior of porphyrin sensitizer is analyzed.

Paper Details

Date Published: 29 December 1997
PDF: 5 pages
Proc. SPIE 3191, Photochemotherapy: Photodynamic Therapy and Other Modalities III, (29 December 1997); doi: 10.1117/12.297824
Show Author Affiliations
Vladimir P. Zorin, Belarussian State Univ. (Belarus)
Ivan I. Khludeyev, Belarussian State Univ. (Belarus)
Valery P. Savitsky, Institute of Radiation Medicine (Belarus)
Sergey B. Mel'nov, Institute of Radiation Medicine (Belarus)
Nina D. Kochubeyeva, Institute of Molecular and Atomic Physics (Belarus)


Published in SPIE Proceedings Vol. 3191:
Photochemotherapy: Photodynamic Therapy and Other Modalities III
Kristian Berg; Benjamin Ehrenberg; Zvi Malik; Johan Moan; Abraham Katzir, Editor(s)

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